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MCQ Questions Class 12 Biotechnology Principles and Processes with Answers - Set - 4
Question 1:
Genes of interest can be selected from a genomic library by using
(a) Restriction enzymes
(b) Cloning vectors
(c) DNA probes
(d) Gene targets
Correct Answer – (A)
Question 2:
Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements.
(i) DNA is negatively charged molecule and so it is loaded on gel towards the anode terminal.
(ii) DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
(iii) Smaller the size of DNA fragment larger is the distance it travels through it.
(iv) Pure DNA can be visualized directly by exposing UV radiation.
Choose correct answer from the options given below.
(a) (i), (iii) and (iv)
(b) (i), (ii) and (iii)
(c) (ii), (iii) and (iv)
(d) (i), (ii) and (iv)
Correct Answer – (D)
Question 3:
In an experiment, recombinant DNA bearing ampicillin-resistance gene is transferred into E.coli cells. The host cells are then cultured on a medium containing ampicillin. The result will be
(a) both transformants and non-transformants cannot survive.
(b) both transformants and non-transformants can survive.
(c) transformants only and not the non-transformants can survive.
(d) transformants cannot survive, but non-transformants can not
Correct Answer – (C)
Question 4:
Identify the wrong statement with regard to restriction enzymes.
(a) Each restriction enzyme functions by inspecting the length of a DNA sequence.
(b) They cut the strand of DNA at palindromic sites.
(c) They are useful in genetic engineering.
(d) Sticky ends can be joined by using DNA ligases.
Correct Answer – (D)
Question 5:
Genetic engineering is possible, because
(a) we can cut DNA at specific sites by endonucleases like DNase I
(b) restriction endonucleases purified from bacteria can be used in vitro
(c) the phenomenon of transduction in bacteria is well understood
(d) we can see DNA by electron microscope.
Correct Answer – (B)
MCQ Questions Class 12 Biotechnology Principles and Processes With Answers
Question 6:
A gene whose expression helps to identify transformed cell is known as
(a) vector
(b) plasmid
(c) structural gene
(d) selectable marker
Correct Answer – (D)
Question 7:
Following statements describe the characteristics of the enzyme restriction endonuclease. Identify the incorrect statement.
(a) The enzyme recognises a specific palindromic nucleotide sequence in the DNA.
(b) The enzyme cuts DNA molecule at identified position within the DNA.
(c) The enzyme binds DNA at specific sites and cuts only one of the two strands.
(d) The enzyme cuts the sugar-phosphate backbone at specific sites on each strand
Correct Answer – (C)
Question 8:
The sequence that controls the copy number of the linked DNA in the vector, is termed
(a) selectable marker
(b) Ori site
(c) palindromic sequence
(d) recognition site.
Correct Answer – (B)
Question 9:
Which of the following statement is not correct about cloning vector ?
(a) ‘Ori’ is a sequence responsible for controlling the copy number of the linked DNA.
(b) Selectable marker selectively permitting the growth of the non-transformants.
(c) In order to link the alien DNA, the vector needs to have single recognition site for the commonly used restriction enzymes.
(d) The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes.
Correct Answer – (B)
Question 10:
Which of the following are used in gene cloning?
(a) Nucleoids
(b) Lomasomes
(c) Mesosomes
(d) Plasmids
Correct Answer – (D)
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